Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-CoV infection.
Identifieur interne : 003086 ( Main/Exploration ); précédent : 003085; suivant : 003087Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-CoV infection.
Auteurs : Lanying Du [République populaire de Chine] ; Guangyu Zhao ; Yongping Lin ; Hongyan Sui ; Chris Chan ; Selene Ma ; Yuxian He ; Shibo Jiang ; Changyou Wu ; Kwok-Yung Yuen ; Dong-Yan Jin ; Yusen Zhou ; Bo-Jian ZhengSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 2008.
Descripteurs français
- KwdFr :
- Administration par voie nasale, Animaux, Cellules BALB 3T3, Dependovirus (génétique), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (administration et posologie), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunité muqueuse, Immunoglobuline A (immunologie), Lymphocytes T cytotoxiques (immunologie), Poumon (immunologie), Production d'anticorps, Protéines de l'enveloppe virale (administration et posologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Rate (immunologie), Réplication virale (), Souris, Syndrome respiratoire aigu sévère (), Vaccination, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- administration et posologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux.
- génétique : Dependovirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux.
- immunologie : Glycoprotéines membranaires, Immunoglobuline A, Lymphocytes T cytotoxiques, Poumon, Protéines de l'enveloppe virale, Rate, Vaccins antiviraux.
- physiologie : Virus du SRAS.
- Administration par voie nasale, Animaux, Cellules BALB 3T3, Glycoprotéine de spicule des coronavirus, Humains, Immunité muqueuse, Production d'anticorps, Réplication virale, Souris, Syndrome respiratoire aigu sévère, Vaccination, Virus du SRAS.
English descriptors
- KwdEn :
- Administration, Intranasal, Animals, Antibody Formation, BALB 3T3 Cells, Dependovirus (genetics), Humans, Immunity, Mucosal, Immunoglobulin A (immunology), Lung (immunology), Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, SARS Virus (drug effects), SARS Virus (physiology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Spleen (immunology), T-Lymphocytes, Cytotoxic (immunology), Vaccination, Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology), Virus Replication (drug effects).
- MESH :
- chemical , administration & dosage : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , immunology : Immunoglobulin A, Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- drug effects : SARS Virus, Virus Replication.
- genetics : Dependovirus.
- immunology : Lung, Spleen, T-Lymphocytes, Cytotoxic.
- physiology : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Administration, Intranasal, Animals, Antibody Formation, BALB 3T3 Cells, Humans, Immunity, Mucosal, Mice, Spike Glycoprotein, Coronavirus, Vaccination.
Abstract
We have previously reported that a subunit protein vaccine based on the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein and a recombinant adeno-associated virus (rAAV)-based RBD (RBD-rAAV) vaccine could induce highly potent neutralizing Ab responses in immunized animals. In this study, systemic, mucosal, and cellular immune responses and long-term protective immunity induced by RBD-rAAV were further characterized in a BALB/c mouse model, with comparison of the i.m. and intranasal (i.n.) routes of administration. Our results demonstrated that: 1) the i.n. vaccination induced a systemic humoral immune response of comparable strength and shorter duration than the i.m. vaccination, but the local humoral immune response was much stronger; 2) the i.n. vaccination elicited stronger systemic and local specific cytotoxic T cell responses than the i.m. vaccination, as evidenced by higher prevalence of IL-2 and/or IFN-gamma-producing CD3+/CD8+ T cells in both lungs and spleen; 3) the i.n. vaccination induced similar protection as the i.m. vaccination against SARS-CoV challenge in mice; 4) higher titers of mucosal IgA and serum-neutralizing Ab were associated with lower viral load and less pulmonary pathological damage, while no Ab-mediated disease enhancement effect was observed; and 5) the vaccination could provide long-term protection against SARS-CoV infection. Taken together, our findings suggest that RBD-rAAV can be further developed into a vaccine candidate for prevention of SARS and that i.n. vaccination may be the preferred route of administration due to its ability to induce SARS-CoV-specific systemic and mucosal immune responses and its better safety profile.
DOI: 10.4049/jimmunol.180.2.948
PubMed: 18178835
Affiliations:
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Zheng</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Antibody Formation</term><term>BALB 3T3 Cells</term><term>Dependovirus (genetics)</term><term>Humans</term><term>Immunity, Mucosal</term><term>Immunoglobulin A (immunology)</term><term>Lung (immunology)</term><term>Membrane Glycoproteins (administration & dosage)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>SARS Virus (drug effects)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spleen (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Vaccination</term><term>Viral Envelope Proteins (administration & dosage)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie nasale</term><term>Animaux</term><term>Cellules BALB 3T3</term><term>Dependovirus (génétique)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (administration et posologie)</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Immunité muqueuse</term><term>Immunoglobuline A (immunologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Poumon (immunologie)</term><term>Production d'anticorps</term><term>Protéines de l'enveloppe virale (administration et posologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Rate (immunologie)</term><term>Réplication virale ()</term><term>Souris</term><term>Syndrome respiratoire aigu sévère ()</term><term>Vaccination</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Immunoglobulin A</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Dependovirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dependovirus</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Immunoglobuline A</term><term>Lymphocytes T cytotoxiques</term><term>Poumon</term><term>Protéines de l'enveloppe virale</term><term>Rate</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lung</term><term>Spleen</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Antibody Formation</term><term>BALB 3T3 Cells</term><term>Humans</term><term>Immunity, Mucosal</term><term>Mice</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie nasale</term><term>Animaux</term><term>Cellules BALB 3T3</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunité muqueuse</term><term>Production d'anticorps</term><term>Réplication virale</term><term>Souris</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccination</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have previously reported that a subunit protein vaccine based on the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein and a recombinant adeno-associated virus (rAAV)-based RBD (RBD-rAAV) vaccine could induce highly potent neutralizing Ab responses in immunized animals. In this study, systemic, mucosal, and cellular immune responses and long-term protective immunity induced by RBD-rAAV were further characterized in a BALB/c mouse model, with comparison of the i.m. and intranasal (i.n.) routes of administration. Our results demonstrated that: 1) the i.n. vaccination induced a systemic humoral immune response of comparable strength and shorter duration than the i.m. vaccination, but the local humoral immune response was much stronger; 2) the i.n. vaccination elicited stronger systemic and local specific cytotoxic T cell responses than the i.m. vaccination, as evidenced by higher prevalence of IL-2 and/or IFN-gamma-producing CD3+/CD8+ T cells in both lungs and spleen; 3) the i.n. vaccination induced similar protection as the i.m. vaccination against SARS-CoV challenge in mice; 4) higher titers of mucosal IgA and serum-neutralizing Ab were associated with lower viral load and less pulmonary pathological damage, while no Ab-mediated disease enhancement effect was observed; and 5) the vaccination could provide long-term protection against SARS-CoV infection. Taken together, our findings suggest that RBD-rAAV can be further developed into a vaccine candidate for prevention of SARS and that i.n. vaccination may be the preferred route of administration due to its ability to induce SARS-CoV-specific systemic and mucosal immune responses and its better safety profile.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chan, Chris" sort="Chan, Chris" uniqKey="Chan C" first="Chris" last="Chan">Chris Chan</name><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><name sortKey="Lin, Yongping" sort="Lin, Yongping" uniqKey="Lin Y" first="Yongping" last="Lin">Yongping Lin</name><name sortKey="Ma, Selene" sort="Ma, Selene" uniqKey="Ma S" first="Selene" last="Ma">Selene Ma</name><name sortKey="Sui, Hongyan" sort="Sui, Hongyan" uniqKey="Sui H" first="Hongyan" last="Sui">Hongyan Sui</name><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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